we can go deeper
Field To Lab 1.0: Fungal Microscopy Boot Camp
with Denise Williams

Field 2 Lab 1.0
with Denise Williams
Date: June 12-14
Location: ThunderBridge
Address: 1425 Arnolds Valley Rd, Natural Bridge Station, VA 24579
Overview
A full-immersion weekend designed to take beginners and intermediate users from “I’m not sure how to start” to confidently preparing slides, using oil immersion, and capturing publication-quality micrographs.
This hands-on workshop is for mushroom enthusiasts with little or no microscope experience, as well as those who want to build confidence and skill. The goal is to remove the intimidation factor from microscopy by teaching practical skills for preparing slides, staining tissues, handling oil immersion, making accurate micro-measurements, and digital imaging. As the weekend progresses, we also move beyond the basics into advanced contrast-enhancement techniques, such as Kohler, darkfield, oblique, Rheinberg, and related methods, to help participants produce striking and informative images that reveal more than standard brightfield microscopy alone.
By the end, attendees leave with the confidence to continue exploring fungal microanatomy on their own, using clear and reproducible methods that support both personal learning and community science.
Microscopes are provided!
Participants without their own microscopes will have access to quality, fully serviced instruments throughout the workshop. These microscopes will also be available for purchase at the end of the event at very reasonable prices, ideal for anyone who wants to continue practicing microscopy at home.
Draft Schedule
Friday Evening
6:30–9:00 pm — The Basics: Setting Up Your Scope (and How Not to Break Stuff)
A practical and hands-on walk-through of microscope components, illumination, focusing, handling objectives, and basic troubleshooting, followed by slide preparation and lots of guided practice.
Saturday Morning
9:00 am–12:00 pm — Field Trip: Collecting Fungal Specimens
A guided walk to gather fresh material to bring back to the lab. We’ll cover sampling technique, how to keep specimens intact for microscopy, and what macroscopic features matter for the microscope later.
Saturday Afternoon
1:00–4:00 pm — Oil Immersion, Measuring, and Digital Imaging
Master the techniques that unlock fungal structures under the microscope at high magnification: contrast stains, oil-immersion work, micrometry, and micrography, followed by practice observing and imaging the morning’s finds.
Saturday Evening
7:00–8:00 pm — Advanced Contrast Enhancement Techniques
Explore Kohler, oblique, darkfield, Rheinberg, polarization, and many other illumination tricks, followed by an open workshop where participants can make their own filters.
Throughout the workshop there will be lots of guided practice with one-on-one help.
Come wander with us.
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